Hyb-Seq: Combining Target Enrichment and Genome Skimming for Plant Phylogenomics
نویسندگان
چکیده
منابع مشابه
Hyb-Seq: Combining target enrichment and genome skimming for plant phylogenomics1
UNLABELLED PREMISE OF THE STUDY Hyb-Seq, the combination of target enrichment and genome skimming, allows simultaneous data collection for low-copy nuclear genes and high-copy genomic targets for plant systematics and evolution studies. • METHODS AND RESULTS Genome and transcriptome assemblies for milkweed (Asclepias syriaca) were used to design enrichment probes for 3385 exons from 768 ge...
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Summary Hybridization-based target enrichment in combination with genome skimming (Hyb-Seq) is becoming a standard method of phylogenomics. We developed HybPhyloMaker, a bioinformatics pipeline that performs target enrichment data analysis from raw reads to supermatrix-, supertree-, and multispecies coalescent-based species tree reconstruction. HybPhyloMaker is written in BASH and integrates co...
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Phylogenetics benefits from using a large number of putatively independent nuclear loci and their combination with other sources of information, such as the plastid and mitochondrial genomes. To facilitate the selection of orthologous low-copy nuclear (LCN) loci for phylogenetics in nonmodel organisms, we created an automated and interactive script to select hundreds of LCN loci by a comparison...
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ژورنال
عنوان ژورنال: Applications in Plant Sciences
سال: 2014
ISSN: 2168-0450
DOI: 10.3732/apps.1400042